“Gene evolution, refers to a technique for artificially accelerating the naturally occurring recombination phenomena in a laboratory of laboratories to select genes with the properties we desire in a short time.”

On looking at the evolutionary process of living things, mutation is firstly introduced into genes by environmental factors.
These genes are repeatedly, genetically engineered in a variety of forms through the recombination process called sexual reproduction.
Under these mutants, if it meets the selection criteria of the natural environment, it will be dominating species, otherwise it will disappear.
In other words, organisms generate genetic diversity through genetic mutation and gene evolution processes, genetic diversity is selected
through selection criteria of the natural environment, and more developed excellent genes are created.
These methods of natural evolution will take millions of years for a given gene to improve in a particular direction.

“Gene evolution” refers to a technique for artificially accelerating the naturally occurring recombination phenomena in a laboratory of laboratories
to select genes with the properties we desire in a short time.”

- By obtaining a group of genes with different mutations and
- Making different recombinant DNA libraries by exchanging different mutations of these gene groups
- It is a technology to select useful genes with the necessary properties from this library.

High added value enzymes and proteins can be replaced with existing cvhemical processes if they need to be manufactured at a lower cost.
In order to achieve this, it is possible to culture the microorganisms in which the enzyme is first expressed, i.e., fermentation by mass production.
It can make mass production at a low cost by maximizing the culture conditions of various microorganisms
such as appropriate culture medium, various nutrients for cultivation, bath pH, culture temperature and oxygen demand.

Enzymes produced by the way of mass fermentation must be separated and purified by various methods, depending on
whether they are expressed intracellularly or secreted on other cells.
When secreted in addition to cells, it is only necessary that cells are removed and only the culture solution is recovered and purified,
but when expressed in the cells, it needs complicated process such as recovery of cells, destruction of cells, destruction of minced cell debris
and removal of various nucleic acid substances to separate and purify with a clean and high purity enzyme.

For the utilization of expensive enzymes and proteins industrially, it is necessary to reuse to save the cost as much as possible in the production process,
which in turn increases competitiveness compared to technology with chemical processes.
In order to reuse water-soluble enzymes and proteins, it is necessary to immobilize them on insoluble carriers to separate them from the reaction products.
In the case of immobilizing the enzyme, it can be easily separated from the reaction product and can be continuously reused.
In particular, it can increase the cycle by apparent increase of the stability due to external conditions such as temperature, pH, pressure, etc.
However, there are disadvantages that the active site of the enzyme is inactivated by deformation or the reaction efficiency reduced due to a decrease in substance diffusion.

The nature of the carrier used for the immobilization and the immobilization method varies depending on the type of enzyme, used protein and the reaction system.
In addition to it, the properties such as carrier size, pore size, affinity, strength and etc depending on the reaction system of the enzyme should be optimized so that the immobilized enzyme can be used efficiently.