The word “Amicogen” consists of the meaning of friend, “AMICO”
in Latin and of gene, “GENE”. We developed innovative biocatalysts
and proteins by applying our proprietary i DNA Evolution™ Technology.
We are a “Bioengineering company just like a Sincere Friend”
who contributes to the human health and the preservation of environment.
Amicogen Biotech R&D Center is expanding its R&D field based on
bio-platform technology, and researchers account for 30% of all employees.
We will lead the Industrial Biotechnology to develop new technologies
that can drive the development of humanity and society,
one step further than others.
Amicogen has developed Special Enzymes for industrial and pharmaceutical products based on the i DNA evolution™.
We want to contribute to the sustainable growth and lifelong health
of humanity by developing New Bio-Ingredientfor Health functional foods
with Special Enzymes and Protein Purification Resin.
As Amicogen started as a bio-venture company, we have focused on
the core competence of R&D and are pursuing sustainable growth
in innovative technologies and products. To increase the value
of enterprise and shareholder, we are continually strengthening our core competencies such as specialty enzyme business, healthcare, protein
purification resin business and others.
Amicogen promotes various activities to realize the value of contributing
to the protection of human health and the environment.
Amicogen is positioning itself as a global biotechnology company
through growth and leaps to create new values.
Amicogen has developed bio innovative technologies and operated the businesses that consider humankind and environment from the perspective of sustainability for the past 20 years. We will continue sustainable development based on Amicogen's environmental soundness, social responsibility and sustainable innovative technology.
Amicogen produces new technologies and values based on
creative thinking and constant technological innovation through human
oriented management. We do our best to ensure that talented individuals
with unique skills and abilities can demonstrate their abilities
according to the nature of their duties.
Amicogen produces new technologies and values based on
creative thinking and constant technological innovation through human
oriented management. We do our best to ensure that talented individuals
with unique skills and abilities can demonstrate their abilities
according to the nature of their duties.
We are developing Bio products and New Bio-Ingredients
using i DNA Evolution™ Technology.
WorkBeads™ 40 Co-NTA
WorkBeads™ 40 NTA resins are based on the nitrilotriacetic acid (NTA) chelating group.
The resin can easily be charged, before use, with a broad spectrum of divalent or
trivalent transition metal ions, including Ni2+, Co2+, Cu2+ and Zn2+, Ga3+ or Fe3+.
They can then be used for the Immobilized Metal Ion Affinity Chromatography (IMAC)
purification of His-tagged proteins or other proteins with an affinity for metal ions.
, which are immobilized via a metal chelating ligand on the chromatography resin.
• Resin charged (or pre-charged) with the metal ion of choice
• High binding capacity and purity. Outstanding flow properties.
• Reliable and reproducible results
• Prepacked ready-to-use columns for rapid and reliable results - see BabyBio IMAC page
Product
The WorkBeads™ NTA resins are based on the nitrilotriacetic acid (NTA) chelating group.
The resin can easily be charged, before use,with a broad spectrum of divalent or trivalent
transition metal ions, including Ni2+, Co2+, Cu2+ or Zn2+, Fe3+ or Ga3+.
They can then be used for the Immobilized Metal Ion Affinity Chromatography (IMAC)
purification of His-tagged proteins or other proteins with an affinity for metal ions.
The selectivity of the metal-charged resin depends on both the choice of ligand (NTA or IDA)
and the metal ion used. The uncharged resins can also be used for divalent metal ion removal.
Main characteristics of WorkBeads 40 Ni-NTA, WorkBeads 40 Co-NTA,
WorkBeads 40 Cu-NTA and WorkBeads 40 Zn-NTA resins.
| WorkBeads 40 Ni-NTA | WorkBeads 40 Co-NTA | WorkBeads 40 Cu-NTA | WorkBeads 40 Zn-NTA |
Target substance | His-tagged proteins, proteins containing histidine cysteine and/or tryptophan amino acid side chains | |||
Matrix | Highly cross-linked | Highly cross-linked | Highly cross-linked | Highly cross-linked |
Average particle size1 | 45 μm | 45 μm | 45 μm | 45 μm |
Chelating ligand | Nitrilotriacetic acid | NTA | NTA | NTA |
Metal ion | Nickel (II) | Cobalt (II) | Cupper (II) | Zink (II) |
Metal ion capacity for the chelating ligand2 | NA | NA | 50 - 60 μmol Cu 2+/ml | NA |
Dynamic binding | > 60 mg HiS6-GFP/ml | NA | NA | NA |
Max flow rate (20 cm | 600 cm/h | 600 cm/h | 600 cm/h | 600 cm/h |
Chemical stability | Compatible with all standard aqueous buffers used for protein purification, | |||
pH stability | 7 - 9 (working range) | 7 - 9 (working range) | 7 - 9 (working range) | 7 - 9 (working range) |
Storage | 2 to 25 °C | 2 to 25 °C | 2 to 25 °C | 2 to 25 °C |
1. The median particle size of the cumulative volume distribution.
2. Metal ion capacity is determined by frontal analysis at 50% breakthrough using cupper solution.
3. The binding capacity is determined using a BabyBio Ni-NTA 1 ml. The binding capacity is dependent on the size of the target protein, and on the competition of impurities.
Product List
Product name | Size/Volume | Article Number |
WorkBeads 40 NTA | Bulk-Media - 25 mL | 40 602 001 |
Bulk-Media - 150 mL | 40 602 003 | |
Bulk-Media - 1 L | 40 602 010 | |
WorkBeads 40 Ni-NTA | Bulk-Media - 25 mL | 40 651 001 |
Bulk-Media - 150 mL | 40 651 003 | |
Bulk-Media - 1 L | 40 651 010 | |
WorkBeads 40 Cu-NTA | Bulk-Media - 25 mL | 40 651 301 |
Bulk-Media - 150 mL | 40 651 303 | |
Bulk-Media - 1 L | 40 651 310 | |
WorkBeads 40 Co-NTA | Bulk-Media - 25 mL | 40 651 401 |
Bulk-Media - 150 mL | 40 651 403 | |
Bulk-Media - 1 L | 40 651 410 | |
WorkBeads 40 Zn-NTA | Bulk-Media - 25 mL | 40 651 501 |
Bulk-Media - 150 mL | 40 651 503 | |
Bulk-Media - 1 L | 40 651 510 |