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About Us
About Us
艾美科健(AMICOGEN)是AMICO(=拉丁语中意为"朋友")+GEN(=Gene,
"基因")的合成词， 公司通过其核心技术即基因进化技术，
致力于成为促进人类可持续发展，
保护地球环境的“真正的朋友”。
艾美科健作为韩国代表性的工业生物技术企业，
致力于为人类健康和环境保护做贡献。 -
R&D
为首次开发出最好的产品，
进行不间断的研究和开发。 -
Products
Products
艾美科健以独家的基因进化技术为基础(i DNA evolution TM)
开发出工业用·制药用特殊酶产品。
利用特殊酶开发出健康功能食品原料即新型生物材料、
蛋白质提炼用树脂，为人类的可持续发展和健康做贡献。
开发新的生物产品
和健康功能食品原料。 -
Investors
Investors
艾美科健从生物科技企业做起，将核心力量关注在R&D上，
以创新的技术和产品追求可持续增长。
为了提高企业价值和股东价值，
在健康保健·蛋白质提炼树脂等领域不断强化核心力量。
为提高企业价值和股东价值，
不断强化核心力量。 -
Media
查看更多关于艾美科键的信息吧。 -
ESG
可持续发展
过去的20年来，艾美科健始终从可持续的观点出发，开发出致力于人类和环境的生物创新技术， 从事经营。
艾美科健将以环境健全性、社会责任性、可持续创新技术为基础，推动企业可持续发展。
艾美科健将以可持续经营为基础，认真履行社会责任. -
Careers
期待创造新价值和
新未来的艾美科健人的加入。 -
CONTACT
期待创造新价值和
新未来的艾美科健人的加入。
蛋白质提炼用树脂
Specialty enzyme
艾美科健以差异化的R&D技术和品质管理能力
开发新的生物产品和健康保健食品原料。
CONTACT USWorkBeads™ 40 IDA resins are based on the iminodiacetic acid (IDA) chelating groups.
The resins can easily be charged, before use, with a broad spectrum of divalent or
trivalent transition metal ions, including Ni2+, Co2+, Cu2+ and Zn2+, Ga3+ or Fe3+.
They can then be used for the Immobilized Metal Ion Affinity Chromatography (IMAC)
purification of His-tagged proteins or other proteins with an affinity for metal ions.
, which are immobilized via a metal chelating ligand on the chromatography resin.
• Resin charged (or pre-charged) with the metal ion of choice
• High binding capacity and purity. Outstanding flow properties.
• Reliable and reproducible results
• Prepacked ready-to-use columns for rapid and reliable results - see BabyBio IMAC page Product
Product
The WorkBeads™ IDA resins are based on the iminodiacetic acid (IDA) chelating group.
The resin can easily be charged, before use,with a broad spectrum of divalent or
trivalent transition metal ions, including Ni2+, Co2+, Cu2+ or Zn2+, Fe3+ or Ga3+.
They can then be used for the Immobilized Metal Ion Affinity Chromatography (IMAC)
purification of His-tagged proteins or other proteins with an affinity for metal ions.
The selectivity of the metal-charged resin depends on both the choice of ligand (NTA or IDA)
and the metal ion used. The uncharged resins can also be used for divalent metal ion removal.
Main characteristics of WorkBeads 40 Ni-IDA, WorkBeads 40 Co-IDA,
WorkBeads 40 Cu-IDA and WorkBeads 40 Zn-IDA resins.
|
WorkBeads 40 Ni-IDA |
WorkBeads 40 Co-IDA |
WorkBeads 40 Cu-IDA |
WorkBeads 40 Zn-IDA |
Target substance |
His-tagged proteins, proteins containing histidine cysteine and/or tryptophan amino acid side chains |
|||
Matrix |
Highly cross-linked |
Highly cross-linked |
Highly cross-linked |
Highly cross-linked |
Average particle size1 |
45 μm |
45 μm |
45 μm |
45 μm |
Chelating ligand |
Iminodiacetic acid |
Iminodiacetic acid |
Iminodiacetic acid |
Iminodiacetic acid |
Metal ion |
Nickel (II) |
Cobalt (II) |
Cupper (II) |
Zink (II) |
Metal ion capacity for the chelating ligand2 |
NA |
NA |
50 - 60 μmol Cu 2+/ml |
NA |
Dynamic binding capacity3 |
> 60 mg His6- |
NA |
NA |
NA |
Max flow rate (20 cm |
600 cm/h |
600 cm/h |
600 cm/h |
600 cm/h |
Chemical stability |
Compatible with all standard aqueous buffers used for protein purification, 8 M urea, 6 M guanidine-HCl, non-ionic detergents, 20% ethanol. Chelating substances (e.g. EDTA) will strip off the metal ions. Stripped resin: 10 mM HCl (pH 2), 10 mM NaOH (pH 12),10 mM sodium citrate-HCl (pH 3). |
|||
pH stability |
7 - 9 (working range) |
7 - 9 (working range) |
7 - 9 (working range) |
7 - 9 (working range) |
Storage |
2 to 25 °C |
2 to 25 °C |
2 to 25 °C |
2 to 25 °C |
1. The median particle size of the cumulative volume distribution.
2. Metal ion capacity is determined by frontal analysis at 50% breakthrough using cupper solution.
3. The binding capacity is determined using a BabyBio Ni-NTA 1 ml, equal value is expected for IDA resins.
Product List
Product name |
Size/Volume |
Article Number |
WorkBeads 40 IDA |
Bulk-Media - 25 mL |
40 601 001 |
Bulk-Media - 150 mL |
40 601 003 |
|
Bulk-Media - 1 L |
40 601 010 |
|
WorkBeads 40 Ni-IDA |
Bulk-Media - 25 mL |
40 650 001 |
Bulk-Media - 150 mL |
40 650 003 |
|
Bulk-Media - 1 L |
40 650 010 |
|
WorkBeads 40 Cu-IDA |
Bulk-Media - 25 mL |
40 650 301 |
Bulk-Media - 150 mL |
40 650 303 |
|
Bulk-Media - 1 L |
40 650 310 |
|
WorkBeads 40 Co-IDA |
Bulk-Media - 25 mL |
40 650 401 |
Bulk-Media - 150 mL |
40 650 403 |
|
Bulk-Media - 1 L |
40 650 410 |
|
WorkBeads 40 Zn-IDA |
Bulk-Media - 25 mL |
40 650 501 |
Bulk-Media - 150 mL |
40 650 503 |
|
Bulk-Media - 1 L |
40 650 510 |