AMICOGEN

단백질 정제 레진

Specialty enzyme

아미코젠은 차별화된 R&D 기술과 품질관리 능력으로
새로운 바이오 제품과 건강기능식품 원료를 개발하고 있습니다.

단백질 정제 레진 - Affinity

ProA™

rProtein A Agarose Resin

Protein A is a surface protein found in cell wall produced by several strains of Staphylococcus aureus
that consists of a single polypeptide chain and contains little or no carbohydrate.
Recombinant Protein A is produced in E. coli and shows essentially the same properties
as native Protein A. Protein A molecule contains five high affinity binding sites capable of binding with
the Fc region from IgG of many species of mammal including human and rabbit. (Fig 1)​

 

 985e0281c5033d5b2e5614350e3acae5_1527151

Fig 1. Recombinant Protein A ligand
S: signal sequence; E,D,A,B,C: IgG binding domain
XM: Cell wall-attaching structure​

 

The interaction between Protein A and IgG is not equivalent for all species.
Even within a species, Protein A interacts with some subclasses of IgG but not others.
For example, human IgG1, IgG2 and IgG4 binds strongly, while IgG3 does not bind.
There are also many examples in which monoclonal antibodies do not bind to Protein A, especially the majority of rat.
Recombinant Protein A has been coupled to 4% highly crosslinked agarose bead.
ProATM displays high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength.
It can be used for IgG purification and immunoprecipitation.​

 

 

 ITEM

 DESCRIPTION

 Matrix

 4% Highly cross-linked agarose

 Ligand

 Recombinant Protein A

 Particle Size

 45-165 μm

 Working Velocity

 30-300 cm/hr

 Binding Capacity

 >28 mg rabbit IgG/ml resin

 Working pH

 3-10

 CIP

 2-13

 Temp. Stability

 2-40°C

 

 

Product List

 Product name

 Size/Volume

 Article Number

 ProA(IP)

 1ml

 1080001

 5ml

 1080005

 25ml

 1080025

 100ml

 1080100

 ProA(정제)

 5ml

 1010005

 25ml

 1010025

 100ml

 1010100

 200ml

 1010200

 1000ml

 1011000

 ProG(IP)

 1ml

 2080001

 5ml

 2080005

 25ml

 2080025

 100ml

 2080100

 ProG(정제)

 5ml

 2010005

 25ml

 2010025

 100ml

 2010100

 200ml

 2010200

 1000ml

 2011000

 ProA/G(IP)

 1ml

 4080001

 5ml

 4080005

 25ml

 4080025

 ProL(정제)

 5ml

 3010005

 25ml

 3010025