단백질 정제 레진

Specialty enzyme

아미코젠은 차별화된 R&D 기술과 품질관리 능력으로
새로운 바이오 제품과 건강기능식품 원료를 개발하고 있습니다.

단백질 정제 레진 - Affinity


rProtein A Agarose Resin

Protein A is a surface protein found in cell wall produced by several strains of Staphylococcus aureus
that consists of a single polypeptide chain and contains little or no carbohydrate.
Recombinant Protein A is produced in E. coli and shows essentially the same properties
as native Protein A. Protein A molecule contains five high affinity binding sites capable of binding with
the Fc region from IgG of many species of mammal including human and rabbit. (Fig 1)​



Fig 1. Recombinant Protein A ligand
S: signal sequence; E,D,A,B,C: IgG binding domain
XM: Cell wall-attaching structure​


The interaction between Protein A and IgG is not equivalent for all species.
Even within a species, Protein A interacts with some subclasses of IgG but not others.
For example, human IgG1, IgG2 and IgG4 binds strongly, while IgG3 does not bind.
There are also many examples in which monoclonal antibodies do not bind to Protein A, especially the majority of rat.
Recombinant Protein A has been coupled to 4% highly crosslinked agarose bead.
ProATM displays high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength.
It can be used for IgG purification and immunoprecipitation.​






 4% Highly cross-linked agarose


 Recombinant Protein A

 Particle Size

 45-165 μm

 Working Velocity

 30-300 cm/hr

 Binding Capacity

 >28 mg rabbit IgG/ml resin

 Working pH




 Temp. Stability




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