AMICOGEN

단백질 정제 레진

Specialty enzyme

아미코젠은 차별화된 R&D 기술과 품질관리 능력으로
새로운 바이오 제품과 건강기능식품 원료를 개발하고 있습니다.

단백질 정제 레진 - Ion-Exchange

WorkBeads™ 100 Q

Agarose-based resin for chromatography

 

The WorkBeads™ 100S and WorkBeads 100Q resins for ion exchange chromatography
are designed for industrial purification that requires high flow rate and low backpressure.
The products are intended for use in the purification of proteins, peptides and oligonucleotides
by utilizing the difference in surface charge.
WorkBeads 100S is a strong cation exchanger with sulfonate ligands.
WorkBeads 100Q is a strong anion exchanger with quaternary amine ligands.

 

• High throughput and scalability
• Reliable and reproducible results
• High chemical stability for easy cleaning-inplace​

 

 

Resin description
WorkBeads are agarose-based chromatographic resins  manufactured using a proprietary method
that results in porous beads with a tight size distribution and exceptional mechanical stability.
Agarose based matrices have been successfully used for decades in biotechnology purification, from research to
production scale, due to their exceptional compatibility with biomolecules including proteins, peptides,
nucleic acids and carbohydrates. WorkBeads resins are designed for separations requiring optimal capacity and purity.
WorkBeads 100S is a strong cation exchange resin derivatized with sulfonates as functional groups.
WorkBeads 100Q is a strong anion exchanger derivatized with quaternary amines as functional groups.​

 

 

Main characteristics of WorkBeads 100S and WorkBeads 100Q resins


 WorkBeads 100S

 WorkBeads 100Q

 Target substances

 Proteins and peptides

 Proteins, peptides and oligonucleotides

 Matrix

 Rigid, highly cross-linked agarose

 Rigid, highly cross-linked agarose

 Average particle size1 (Dv50)

 90 - 110 μm

 90 - 110 μm

 Ionic group (ligand)

 Sulfonate (-SO3-)

 Quarternary amine (-N+(CH3)3)

 Ionic capacity

 180 - 250 μmol H+/ml resin

 140 - 200 μmol Cl-/ml resin

 Dynamic binding capacity (DBC)

 >100 mg BSA/ml resin2

 >40 mg BSA/ml resin3

 Pressure flow characteristic

 2 bar at 900 cm/h, 25 mm diameter column, 20 cm bed height

 Chemical stability

 Compatible with all standard aqueous buffers used for protein purification,
 1M NaOH, 30% isopropanol or 70% ethanol. Should not be stored at < pH3
 for prolonged time​

 pH stability

 2 - 13

 2 - 13

 Storage

 2 to 25°C in 20% ethanol

 2 to 25°C in 20% ethanol

 

1. The median particle size of the cumulative volume distribution.
2. Dynamic binding capacity determined at 4-minutes residence time in the presence of 20 mM Na-citrate, pH 4.0.
3. Dynamic binding capacity determined at 4-minutes residence time in the presence of 50 mM Tris-HCl, 50 mM NaCl, pH 8.0.​

 

 

Product List

 Product name

 Size/Volume

 Article Number

 WorkBeads 100 Q

 Bulk-Media - 25 mL

 10 100 001

 Bulk-Media - 200 mL

 10 100 002

 Bulk-Media - 1 L

 10 100 010

 WorkBeads 100 S

 Bulk-Media - 25 mL

 10 200 001

 Bulk-Media - 200 mL

 10 200 002

 Bulk-Media - 1 L

 10 200 010