AMICOGEN

특수효소

Specialty enzyme

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특수효소 - 산업용

TL-10X

Thermomyces lanuginosus lipase (E.C. 3.1.1.3)

Description
TL-10X is a 1,3-specific lipase originating from Thermomyces lanuginosus, immobilized on hydrophobic carrier

 

 

Standard

 Activitya

 >4,000 Propyl Laurate Unit (PLU)/g (dry weight)

 Color

 White

 Properties

 Dried immobilized enzyme

 Size of immobilization support

 200~300 ㎛(>95%)

 Shape

 Spherical type

 Water content

 <5%

 Enzyme assay

 a1 PLU is the amount of enzyme to produce 1ol propyl laurate per min at 60℃ by utilizing lauric acid and 1-propanol as substrates

 

 

 

Application
· Esterification, de-esterification, and interesterification
· Biodiesel production
· Emulsifier making

 

 

Storage conditions
· No direct light
· Dry condition
· Temperature maintenance (4~10°C)​

 

 

Characteristics
· High stability
· High activity toward diverse substrates​

 

 

Condition for transesterification of fats

 Optimal ratio of enzyme

 ~10% of substrate weight

 Type of substrate

 Fats, cooking oil etc.

 Ratio of methanol

 Depending on the types of substrate and reactor

 Optimal temperature

 40℃~50℃

 

 

 

Reaction instruction

 Step

 Experimental procedure

 1

 Pour 1g TL-10X in 15mL conical tube.

 2

 Mix with 9mL substrate(soybean oil)b.

 3

 Locate in shaking incubator at 40℃, 200 rpm.

 4

 After pouring 0.3mL methanolc,add 0.2mL every hour.

 5

 Finish reaction after 6 hours.

 

b The ratio of oil to methanol is depending on the types of oils.

c If methanol is in direct contact with the enzyme or

if it is added in excess of the standard, the enzyme activity may be reduced.

 

 

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·The performance of TL-10X is better than TL lipase from N company over time  since beads of TL-10X are more rigid and stable.

·Transesterification using soy oil and methanol was done with 10% immobilized enzyme at 40℃.

 

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· TL-10X can be used for 1 month in continuous reactor.

· Transesterification using soy oil and methanol was done with 10% immobilized enzyme at 40℃