AMICOGEN

특수효소

Specialty enzyme

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특수효소 - 산업용

CalB-10X

Candida antarctica lipase B (E.C. 3.1.1.3)

Description
CalB-10X is a lipase B originating from candida antarctica, immobilized on hydrophobic carrier.

 

 

Standard

 Activitya

 >5,000 Propyl Laurate Unit(PLU)/g (dry weight)

 Color

 White

 Properties

 Dried immobilized enzyme

 Size of immobilization support

 200~300 ㎛ (>95%)

 Shape

 Spherical type

 Water content

 <5%

 Enzyme assay

 a1 PLU is the amount of enzyme to produce 1ol propyl laurate per min at 60℃ by utilizing lauric acid and 1-propanol as substrates

 

 

 

Application

· Esterification, de-esterification, and interesterification
· Biodiesel production
· Food making​

 

 

Storage conditions
· No direct light
· Dry condition
· Temperature maintenance (4~10°C)

 

 

Characteristics
· High stability
· High activity toward diverse substrates
· High activity up to 5% water content in substrates​

 

 

Condition for transesterification of fats

 Optimal ratio of enzyme

 ~10% of substrate weight

 Type of substrate

 Fats, cooking oil, waste cooking oil, sludge oil etc.

 Ratio of methanol

 Depending on the types of substrate and reactor

 Optimal temperature

 40℃

 

 

 

Reaction instruction

 Step

 Experimental procedure

 1

 Pour 1g CalB-10X in 15mL conical tube.

 2

 Mix with 9mL substrate(soybean oil)b.

 3

 Locate in shaking incubator at 40℃, 200 rpm.

 4

 After pouring 0.3mL methanolc, add 0.2mL every hour.

 5

 Finish reaction after 6 hours.

 

bThe ratio of oil and methanol is changed according to the types of oils. 

cThe enzyme activity decreases if methanol contacts directly with enzyme or methanol is added as more than standard.

 


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