Specialty enzyme

아미코젠은 차별화된 R&D 기술과 품질관리 능력으로
새로운 바이오 제품과 건강기능식품 원료를 개발하고 있습니다.

특수효소 - 산업용


Candida antarctica lipase B (E.C.

CalB-10X is a lipase B originating from candida antarctica, immobilized on hydrophobic carrier.





 >5,000 Propyl Laurate Unit(PLU)/g (dry weight)




 Dried immobilized enzyme

 Size of immobilization support

 200~300 ㎛ (>95%)


 Spherical type

 Water content


 Enzyme assay

 a1 PLU is the amount of enzyme to produce 1ol propyl laurate per min at 60℃ by utilizing lauric acid and 1-propanol as substrates





· Esterification, de-esterification, and interesterification
· Biodiesel production
· Food making​



Storage conditions
· No direct light
· Dry condition
· Temperature maintenance (4~10°C)



· High stability
· High activity toward diverse substrates
· High activity up to 5% water content in substrates​



Condition for transesterification of fats

 Optimal ratio of enzyme

 ~10% of substrate weight

 Type of substrate

 Fats, cooking oil, waste cooking oil, sludge oil etc.

 Ratio of methanol

 Depending on the types of substrate and reactor

 Optimal temperature





Reaction instruction


 Experimental procedure


 Pour 1g CalB-10X in 15mL conical tube.


 Mix with 9mL substrate(soybean oil)b.


 Locate in shaking incubator at 40℃, 200 rpm.


 After pouring 0.3mL methanolc, add 0.2mL every hour.


 Finish reaction after 6 hours.


bThe ratio of oil and methanol is changed according to the types of oils. 

cThe enzyme activity decreases if methanol contacts directly with enzyme or methanol is added as more than standard.