AMICOGEN

특수효소

Specialty enzyme

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특수효소 - 산업용

Nuclease SM

Non-specific Endonuclease (E.C. 3.1.30.2)

Description
It is derived from Serratia marcescens and removes nucleic acid(DNA, RNA).

 

 

Standard

 Activitya

 150,000~300,000 U/g

 Properties

 White or yellow colored, freeze-dried powder

 Arsenic(as As2O3)

 < 4 ppm

 Pb

 < 10 ppm

 Heavy metals

 < 40 ppm

 Coliform bacteria group

 < 30/g

 Salmonella

 Negative

 Enzyme assay

 aOne unit (U) represents the amount of enzyme that increases absorbance by 1.0 at 260 nm when it reacts at 37℃, pH 8.2

 

 

 

Application
Removal of nucleic acid (DNA, RNA)

 

 

Storage temperature
Below 4°C

 

 

Characteristics
· Type of nuclease: Non-specific endonuclease
· Optimal temperature: 37°C
· Optimal pH: 6~8
· The effect of metal ion: Mg2+ or Mn2+ are needed for enzyme activity​

 

 

Reaction scheme of enzyme

70bf0e550e6a0555e9784a8f1ff7a99e_1526878

 

 

Optimal reaction conditions
(Temperature, pH)

70bf0e550e6a0555e9784a8f1ff7a99e_1526878