AMICOGEN

특수효소

Specialty enzyme

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특수효소 - 제약용

AMK-EX

Immobilized cephalosporin C or 7-ACA esterase

Description
It produces D-CPC(deacetyl-cephalosporic C) from CPC(Cephalosporin C) or

D-7-ACA(deacetyl-7-aminocephalosporanic acid) from 7-ACA(7-aminocephalosporanic acid).

 

 

Standard

 Activitya

 300 U/g wet weight

 Size of immobilization support

 150~300μm (>95%)

 Water content

 62%

 Enzyme assay

a One unit (U) represents the amount of enzyme required for producing 1μmol D-CPC and acetic acid per min in 50mM K-phosphate buffer containing 5% CPC, pH 8.0, at 25℃.

 

 

 

Application

·D-CPC production from CPC

·D-7-ACA production from 7-ACA

 

 

Storage temperature

4~10°C

 

 

Characteristics
·Very high specific activity
·High substrate selectivity
·High stability (reusable more than 800 cycles)
·High production rate of D-CPC (more than 98%)
·High production rate of D-7-ACA (more than 98%)​

 

 

Reaction condition

 pH

 7.9~8.0

 Temperature

 20°C

 Solvent

 Water

 Enzyme amount

 7~10 U/ml

 Reaction time

 1~1.5 hours

 

 

 

Scheme for enzyme reaction

673c2f371d2ded79a1a5a62819b7d86e_1526629 

 

 

Optimal reaction conditions
(Temperature, pH)

673c2f371d2ded79a1a5a62819b7d86e_1526629