AMICOGEN

특수효소

Specialty enzyme

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특수효소 - 제약용

AMK518

Immobilized cefradine acylase

Description
It produces cefradine from 7-ADCA(7-amino-3-deacetoxy-cephalosporanic acid) and DHME(D-dihydrophenylglycine methyl ester)

 

 

Standard

 Activitya

 100 U/g

 Size of immobilization support

 300~500㎛(>98%)

 Water content

 <60%

 Enzyme assay

 aOne unit (U) represents the amount of enzyme required for producing 1㎛ol cefradine per min in 0.1M K-phosphate buffer containing 20mM 7-ADCA(7-amino-3-deacetoxy-cephalosporanic acid) and 20mM DHME(D-dihydrophenylglycine methyl ester), pH 7.5 at 28℃.

 

 

Application
Cefradine production from 7-ACDA and DHME

 

 

Storage temperature
4~10°C

 

 

Characteristics
·High synthesis activity of cefradine
·Low degradation activity of cefradine and DHME
·Fast enzymatic reaction (reaction completed in 90 mins
·High yield synthesis of cefradine (>95%)​

 

 

Reaction condition

 pH

 8.0~8.3

 Temperature

 10°C

 Solvent

 Water

 7ADCA : PGM

 1:1.05-1.10

 Enzyme amount 

 7U/ml

 

 

 

Scheme for enzyme reaction

673c2f371d2ded79a1a5a62819b7d86e_1526626 

 

 

Optimal reaction conditions

(Temperature, pH)

673c2f371d2ded79a1a5a62819b7d86e_1526626