AMICOGEN

Protein Purification Resin

Specialty enzyme

We are developing Bio products and New Bio-Ingredients
using   i  DNA Evolution™ Technology.

Protein Purification Resin - Immobilized Metal

WorkBeads™ 40 Co-NTA

Agarose-based resin for chromatography

 

WorkBeads™ 40 NTA resins are based on the nitrilotriacetic acid (NTA) chelating group.
The resin can easily be charged, before use, with a broad spectrum of divalent or
trivalent transition metal ions, including Ni2+, Co2+, Cu2+ and Zn2+, Ga3+ or Fe3+.
They can then be used for the Immobilized Metal Ion Affinity Chromatography (IMAC)
purification of His-tagged proteins or other proteins with an affinity for metal ions.
, which are immobilized via a metal chelating ligand on the chromatography resin.

• Resin charged (or pre-charged) with the metal ion of choice
• High binding capacity and purity. Outstanding flow properties.
• Reliable and reproducible results
• Prepacked ready-to-use columns for rapid and reliable results - see BabyBio IMAC page

 

 

Product
The WorkBeads™ NTA resins are based on the nitrilotriacetic acid (NTA) chelating group.
The resin can easily be charged, before use,with a broad spectrum of divalent or trivalent
transition metal ions, including Ni2+, Co2+, Cu2+ or Zn2+, Fe3+ or Ga3+.

 

They can then be used for the Immobilized Metal Ion Affinity Chromatography (IMAC)

purification of His-tagged proteins or other proteins with an affinity for metal ions.
The selectivity of the metal-charged resin depends on both the choice of ligand (NTA or IDA)
and the metal ion used. The uncharged resins can also be used for divalent metal ion removal.​

 

 

Main characteristics of WorkBeads 40 Ni-NTA, WorkBeads 40 Co-NTA,
WorkBeads 40 Cu-NTA and WorkBeads 40 Zn-NTA resins. 

 

 WorkBeads 40 Ni-NTA

 WorkBeads 40 Co-NTA

 WorkBeads 40 Cu-NTA

 WorkBeads 40 Zn-NTA

 Target substance

 His-tagged proteins, proteins containing histidine cysteine and/or tryptophan amino acid side chains

 Matrix

 Highly cross-linked
agarose

 Highly cross-linked
agarose

 Highly cross-linked
agarose

 Highly cross-linked
agarose

 Average particle size1
 (DV50)

 45 μm

 45 μm

 45 μm

 45 μm

 Chelating ligand

 Nitrilotriacetic acid
(NTA)

 NTA

 NTA

 NTA

 Metal ion

 Nickel (II)

 Cobalt (II)

 Cupper (II)

 Zink (II)

 Metal ion capacity for

 the chelating ligand2

 NA

NA

 50 - 60 μmol Cu 2+/ml
resin

NA

 Dynamic binding
 capacity3

 > 60 mg HiS6-GFP/ml
resin

 NA

NANA

 Max flow rate (20 cm
 bed height and 5 bar)

 600 cm/h

600 cm/h600 cm/h600 cm/h

 Chemical stability

 Compatible with all standard aqueous buffers used for protein purification,
 8 M urea,  6 M guanidine-HCl, non-ionic detergents, 20% ethanol.
 Chelating substances (e.g. EDTA) will strip off the metal ions.
 Stripped resin: 10 mM HCl (pH 2), 10 mM NaOH (pH 12), 10 mM sodium citrate-HCl (pH 3).​

 pH stability

 7 - 9 (working range)
 2 - 12 cleaning
 (stripped resin)​

  7 - 9 (working range)
  2 - 12 cleaning
  (stripped resin)​

  7 - 9 (working range)
  2 - 12 cleaning
  (stripped resin)​

  7 - 9 (working range)
  2 - 12 cleaning
 (stripped resin)​

 Storage

 2 to 25 °C

 2 to 25 °C

 2 to 25 °C

 2 to 25 °C

1. The median particle size of the cumulative volume distribution.
2. Metal ion capacity is determined by frontal analysis at 50% breakthrough using cupper solution.
3. The binding capacity is determined using a BabyBio Ni-NTA 1 ml. The binding capacity is dependent on the size of the target protein, and on the competition of impurities.​


Product List

 Product name

 Size/Volume

 Article Number

 WorkBeads 40 NTA

 Bulk-Media - 25 mL

 40 602 001

 Bulk-Media - 150 mL

 40 602 003

 Bulk-Media - 1 L

 40 602 010

 WorkBeads 40 Ni-NTA

 Bulk-Media - 25 mL

 40 651 001

 Bulk-Media - 150 mL

 40 651 003

 Bulk-Media - 1 L

 40 651 010

 WorkBeads 40 Cu-NTA

 Bulk-Media - 25 mL

 40 651 301

 Bulk-Media - 150 mL

 40 651 303

 Bulk-Media - 1 L

 40 651 310

 WorkBeads 40 Co-NTA

 Bulk-Media - 25 mL

 40 651 401

 Bulk-Media - 150 mL

 40 651 403

 Bulk-Media - 1 L

 40 651 410

 WorkBeads 40 Zn-NTA

 Bulk-Media - 25 mL

 40 651 501

 Bulk-Media - 150 mL

 40 651 503

 Bulk-Media - 1 L

 40 651 510