AMICOGEN

Protein Purification Resin

Specialty enzyme

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Protein Purification Resin - Ion-Exchange

WorkBeads™ 40Q

Agarose-based resin for chromatography

The WorkBeads™ 40 S and WorkBeads 40 Q resins for ion exchange chromatography are designed for research

and industrial scale purification of proteins, peptides and oligonucleotides by utilizing the difference in their surface charge.

WorkBeads 40 S resin is a strong cation exchanger derivatized with sulfonate ligands, and WorkBeads 40 Q resin

is a strong anion exchanger derivatized with quaternary amine ligands.

These resins demonstrate the property of high-resolution separation while giving low backpressure facilitate

both capture and polishing purification applications in standard bioprocess columns.

 

• High throughput and purity.

• Reliable and reproducible results.

• High chemical stability for easy cleaning-in-place.

 

 

Resin description

WorkBeads are agarose-based chromatographic resins manufactured using a proprietary method

that results in porous beads with a tight size distribution and exceptional mechanical stability.

Agarose based matrices have been successfully used for decades in biotechnology purification,

from research to production scale , due to their exceptional compatibility with biomolecules including proteins, peptides,

nucleic acids and carbohydrates. WorkBeads resins are designed for separations requiring optimal capacity and purity.

WorkBeads 40 S is a strong cation exchange resin derivatized with sulfonates as functional groups.

WorkBeads 40 Q is a strong anion exchanger derivatized with quarternary amines as functional groups.

 

 

Main characteristics of the WorkBeads 40 S and WorkBeads 40 Q resins

 

 WorkBeads 40 S

 WorkBeads 40 Q

 Target substances

 Proteins and peptides

 Proteins, peptides and oligonucleotides

 Matrix

 Rigid, highly cross-linked agarose

 Average particle size1 (Dv50)

 45 μm

 Ionic group (ligand)

 Sulfonate (-SO3-)

 Quarternary amine (-N+(CH3)3)

 Ionic capacity

 0.18 - 0.25 mmol H+/ml resin

 0.18 - 0.25 mmol Cl-/ml resin

 Dynamic binding capacity (DBC)

 130 mg BSA/ ml resin2

 47 mg BSA/ml resin3

 Max flow rate

(20 cm bed height and 5 bar)

 600 cm/h

 Chemical stability

 Compatible with all standard aqueous buffers used for protein purification,

 1 M NaOH, 30% isopropanol or 70% ethanol. Should not be stored at < pH 3 for

 prolonged time

 pH stability

 2 - 13

 Storage

 2 to 25°C in 20% ethanol

 

1. The median particle size of the cumulative volume distribution.
2. Dynamic binding capacity determined at 4-minutes residence time in the presence of 20 mM Na-citrate, pH 4.0.
3. Dynamic binding capacity determined at 2.5-minutes residence time in the presence of 50 mM Tris-HCl, 50 mM NaCl, pH 8.0.​

 

 

Product List

 Product name

 Size/Volume

 Article Number

 WorkBeads 40 Q

 Bulk-Media - 25 mL

 40 100 001

 Bulk-Media - 200 mL

 40 100 002

 Bulk-Media - 1 L

 40 100 010

 WorkBeads 40 S

 Bulk-Media - 25 mL

 40 200 001

 Bulk-Media - 200 mL

 40 200 002

 Bulk-Media - 1 L

 40 200 010