AMICOGEN

Protein Purification Resin

Specialty enzyme

We are developing Bio products and New Bio-Ingredients
using   i  DNA Evolution™ Technology.

Protein Purification Resin - Size Exclusion

WorkBeads™ 40/100 SEC

Agarose-based resin for chromatography

WorkBeads™ 40/100 SEC, WorkBeads 40/1000 SEC & WorkBeads 40/10 000 SEC are high performance

size exclusion chromatography media for laboratory and large scale separation of proteins.

Made from agarose, well established and well-known in the biotech industry.


• Excellent Resolution
• Robust Separation Results Can be Achieved Across a Wide Range of Proteins & Conditions
• Chemicall stable for Cleaning in place

The WorkBeads SEC resins allows purifications over a large range of molecular weights.
The chemical resistance of the resins allow purification over a broad range of conditions.
suitable for both lab-scale and process-scale separations in standard columns from low to high flow rates.
The chemical resistance of the resins allow purification over a broad range of conditions.​

 

 

Resin Description

WorkBeads are agarose-based chromatographic resins manufactured by a proprietary method
separations that require optimal purity and flow properties.
Agarose based matrices have been successfully used for decades in biotechnology research from
laboratory to production scale, due to their exceptional compatibility with biomolecules
including proteins, peptides, nucleic acids and carbohydrates. WorkBeads resins are designed for
separations that require optimal purity and flow properties.​

 

 

Main characteristics of WorkBeads SEC resins

 

 WorkBeads

 40/100 SEC

 WorkBeads

 40/1000 SEC

 WorkBeads

 40/10 000 SEC

 Matrix

 Rigid, highly cross-linked agarose

 Rigid, highly cross-linked
agarose

 Rigid, highly cross-linked
agarose

 Separation range1

 10 - 150 kD

 10 - 1200 kD

 20 - 10 000 kD

 Exclusion limit

 150 kD

 1200 kD

 10 000 kD

 Average particle size2 (DV50)

 45 μm

 45 μm

 45 μm

 Recommended flow rate

 20 - 100 cm/h

 20 - 100 cm/h

 20 - 50 cm/h

 Max flow rate3, 4

 300 cm/h (600 cm/h)

 300 cm/h (600 cm/h)

 300 cm/h (600 cm/h)

 Chemical stability

 Compatible with all standard aqueous buffers used for protein purification.
 Should not be stored at low pH for prolonged time.

 pH stability

 2 - 13

 2 - 13

 2 - 13

 Storage in 20% ethanol

 2 to 25 °C

 2 to 25 °C

 2 to 25 °C

 

1. Globular proteins.
2. The median particle size of the cumulative volume distribution.
3. 15 mm (i.d.) x 900 mm column, or 25 x 200 mm (values within brackets)
4. Note: Make sure that the column hardware max pressure is not exceeded.​ 

 

 

Product List

 Product name

 Size/Volume

 Article Number

 WorkBeads 40/100 SEC

 Bulk - Media 25 mL

 40 340 001

 Bulk - Media 300 mL

 40 340 003

 Bulk - Media 1 L

 40 340 010

 WorkBeads 40/1000 SEC

 Bulk - Media 25 mL

 40 300 001

 Bulk - Media 300 mL

 40 300 003

 Bulk - Media 1 L

 40 300 010

 WorkBeads 40/10 000 SEC

 Bulk - Media 25 mL

 40 350 001

 Bulk - Media 300 mL

 40 350 003

 Bulk - Media 1 L

 40 350 010