AMICOGEN

蛋白质提炼用树脂

Specialty enzyme

艾美科健以差异化的R&D技术和品质管理能力
开发新的生物产品和健康保健食品原料。

蛋白质提炼用树脂 - Immobilized Metal

WorkBeads™ 40 IDA

Agarose-based resin for chromatography

WorkBeads™ 40 IDA resins are based on the iminodiacetic acid (IDA) chelating groups.
The resins can easily be charged, before use, with a broad spectrum of divalent or
trivalent transition metal ions, including Ni2+, Co2+, Cu2+ and Zn2+, Ga3+ or Fe3+.
They can then be used for the Immobilized Metal Ion Affinity Chromatography (IMAC)
purification of His-tagged proteins or other proteins with an affinity for metal ions.
, which are immobilized via a metal chelating ligand on the chromatography resin.

 

• Resin charged (or pre-charged) with the metal ion of choice
• High binding capacity and purity. Outstanding flow properties.
• Reliable and reproducible results
• Prepacked ready-to-use columns for rapid and reliable results - see BabyBio IMAC page Product​

 

 

Product

The WorkBeads™ IDA resins are based on the iminodiacetic acid (IDA) chelating group.
The resin can easily be charged, before use,with a broad spectrum of divalent or
trivalent transition metal ions, including Ni2+, Co2+, Cu2+ or Zn2+, Fe3+ or Ga3+.

 

They can then be used for the Immobilized Metal Ion Affinity Chromatography (IMAC)
purification of His-tagged proteins or other proteins with an affinity for metal ions.
The selectivity of the metal-charged resin depends on both the choice of ligand (NTA or IDA)
and the metal ion used. The uncharged resins can also be used for divalent metal ion removal.​

 

 

Main characteristics of WorkBeads 40 Ni-IDA, WorkBeads 40 Co-IDA,
WorkBeads 40 Cu-IDA and WorkBeads 40 Zn-IDA resins.

 

 WorkBeads 40 Ni-IDA

 WorkBeads 40 Co-IDA

 WorkBeads 40 Cu-IDA

 WorkBeads 40 Zn-IDA

 Target substance

 His-tagged proteins, proteins containing histidine cysteine and/or tryptophan amino acid side chains

 Matrix

 Highly cross-linked
agarose

 Highly cross-linked
agarose

 Highly cross-linked
agarose

 Highly cross-linked
agarose

 Average particle size1
(DV50)

 45 μm

 45 μm

 45 μm

 45 μm

 Chelating ligand

 Iminodiacetic acid
(IDA)

 Iminodiacetic acid
(IDA)

 Iminodiacetic acid
(IDA)

 Iminodiacetic acid
(IDA)

 Metal ion

 Nickel (II)

 Cobalt (II)

 Cupper (II)

 Zink (II)

 Metal ion capacity for 

 the chelating ligand2

 NA

 NA

 50 - 60 μmol Cu 2+/ml
resin

 NA

 Dynamic binding

 capacity3

 > 60 mg His6-
GFP/ml resin

 NA

 NA

 NA

 Max flow rate (20 cm
 bed height and 5 bar)

 600 cm/h

 600 cm/h

 600 cm/h

 600 cm/h

 Chemical stability

 Compatible with all standard aqueous buffers used for protein purification, 8 M urea, 6 M guanidine-HCl, non-ionic detergents, 20% ethanol. Chelating substances (e.g. EDTA) will strip off the metal ions. Stripped resin: 10 mM HCl (pH 2), 10 mM NaOH (pH 12),10 mM sodium citrate-HCl (pH 3).

 pH stability

 7 - 9 (working range)
 2 - 12 cleaning
 (stripped resin)​

 7 - 9 (working range)
 2 - 12 cleaning
 (stripped resin)​

 7 - 9 (working range)
 2 - 12 cleaning
 (stripped resin)​

 7 - 9 (working range)
 2 - 12 cleaning
 (stripped resin)​

 Storage

 2 to 25 °C

 2 to 25 °C

 2 to 25 °C

 2 to 25 °C

1. The median particle size of the cumulative volume distribution.
2. Metal ion capacity is determined by frontal analysis at 50% breakthrough using cupper solution.
3. The binding capacity is determined using a BabyBio Ni-NTA 1 ml, equal value is expected for IDA resins.​

 

 

Product List

 Product name

 Size/Volume

 Article Number

 WorkBeads 40 IDA

 Bulk-Media - 25 mL

 40 601 001

 Bulk-Media - 150 mL

 40 601 003

 Bulk-Media - 1 L

 40 601 010

 WorkBeads 40 Ni-IDA

 Bulk-Media - 25 mL

 40 650 001

 Bulk-Media - 150 mL

 40 650 003

 Bulk-Media - 1 L

 40 650 010

 WorkBeads 40 Cu-IDA

 Bulk-Media - 25 mL

 40 650 301

 Bulk-Media - 150 mL

 40 650 303

 Bulk-Media - 1 L

 40 650 310

 WorkBeads 40 Co-IDA

 Bulk-Media - 25 mL

 40 650 401

 Bulk-Media - 150 mL

 40 650 403

 Bulk-Media - 1 L

 40 650 410

 WorkBeads 40 Zn-IDA

 Bulk-Media - 25 mL

 40 650 501

 Bulk-Media - 150 mL

 40 650 503

 Bulk-Media - 1 L

 40 650 510