AMICOGEN

단백질 정제 레진

Specialty enzyme

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단백질 정제 레진 - OptioBio

OptioBio™ 40S 10x100

Agarose-based resin prepacked in chromatography column delivered in 20% ethanol.
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Prepacked OptioBio™ 40S 10x100 glass columns are designed for small-scale purification

as well as screening and optimization in bioprocess development and scale-up.


• Prepacked for reliable and reproducible results
• Optimal for high-performance small-scale purification and method optimization in bioprocess development
• High throughput and purity​

 

 

Product
OptioBio 40S 10x100 column is prepacked with WorkBeads™ 40S resin for ion exchange chromatography (IEX).
The resin is designed for research and industrial scale purification of proteins,
peptides and nucleic acids and utilise the difference in their surface charge.

WorkBeads 40S is a strong cation exchange resin with sulfonate ligands.

WorkBeads are agarose based chromatographic resins manufactured using a proprietary method
that results in porous beads with a tight size distribution and high mechanical stability.
WorkBeads resins are designed for separations that require optimal capacity and purity.

 

 

Main characteristics of OptioBio 40S 10x100 and OptioBio 40Q 10x100 columns

 

OptioBio 40S 10x100

 OptioBio 40Q 10x100 

 Target substance

 Proteins and peptides

 Protein, peptides and oligonucleotides

 Resin

 WorkBeads 40S

 

 Matrix

 Rigid, highly cross-linked agarose

 Rigid, highly cross-linked agarose

 Average particle size (DV50)1

 45 μm

 45 μm

 Ionic group (ligand)

 Sulfonate (-SO3- )

 Quarternary amine (-N+(CH3)3)

 Ionic capacity

 0.18 - 0.25 mmol Na+/ml resin

 0.18 - 0.25 mmol Cl-/ml resin

 Dynamic binding capacity (DBC)

 150 mg BSA/ml resin2

 47 mg BSA/ml resin3

 Column volume (CV)

 7.9 ml

 7.9 ml

 Column dimension

 10 x 100 mm

 6 ml/min (450 cm/h)

 Recommended flow rate

 2 - 4 ml/min (150 - 300 cm/h)

 2 - 4 ml/min (150 - 300 cm/h)

 Maximum flow rate4

 6 ml/min (450 cm/h)

 6 ml/min (450 cm/h)

 Column hardware pressure limit

 2.1 MPa, 21 bar, 305 psi

 2.1 MPa, 21 bar, 305 psi

 Chemical stability

 Compatible with all standard buffers used for protein purification, 1 M NaOH, 30 % isopropanol or 70 % ethanol. Should not be stored at < pH 3 for prolonged time.

 pH stability

 3 - 12 (working range)
 2 - 13 (cleaning)

 3 - 12 (working range)
 2 - 13 (cleaning)

 Storage

 2 to 25 °C in 20% ethanol

 2 to 25 °C in 20% ethanol

1. The median particle size of the cumulative volume distribution.
2. Dynamic binding capacity determined in 20 mM Na-citrate, pH 4.0, at a flow of 2 ml/min (150 cm/h; 4 minutes residence time).
3. Dynamic binding capacity determined in 50 mM Tris-HCl, 50 mM NaCl, pH 8.0, at a flow of 2 ml/min (150 cm/h; 4 minutes residence time).
4. Maximum flow rate for aqueous buffers at 20 °C. Decrease the maximum flow rate if the liquid has a higher viscosity. Higher viscosities can be caused by low temperature. Use half of the maximum flow rate for 20% ethanol.

 

 

Product List

 Product name

 Size/Volume

 Article Number

 OptioBio 40Q 10x100

 7.9ml, Single

 55 410 011

 OptioBio 40S 10x100

 7.9ml, Single

 55 420 011

 

  

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