AMICOGEN

단백질 정제 레진

Specialty enzyme

아미코젠은 차별화된 R&D 기술과 품질관리 능력으로
새로운 바이오 제품과 건강기능식품 원료를 개발하고 있습니다.

단백질 정제 레진 - Ion-Exchange

WorkBeads 40 TREN

Agarose-based resin for chromatography

WorkBeads™ 40 TREN resin for multimodal Ion Exchange Chromatography (IEX)
has a ligand that is positively charged below approx. pH 9.
The resin can be used for several different applications, e.g., for multimodal IEX,
for sample cleanup in monoclonal antibody (MAb) purification processes
in order to guard the Protein A column from chromatins and other host cell impurities,
or as a polishing step in the MAb purification process.

 

• Improved selectivities through multimodal IEX separation
• Reduced fouling of Protein A resins by chromatin and host cell impurity removal
• High binding capacity and purity​ 

 

 

Resin description
WorkBeads are agarose-based chromatographic resins manufactured using a proprietary method
that results in porous beads with a tight size distribution and high mechanical stability.
Agarose based matrices have been successfully used for decades in biotechnology purification,
from research to production scale, due to their exceptional compatibility with biomolecules
including proteins, peptides, nucleic acids and carbohydrates.
WorkBeads resins are designed for separations requiring optimal capacity and purity.​

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WorkBeads 40 TREN resin contains ligands based on Tris(2-aminoethyl)amine (TAEA).
The structure of the ligand used in WorkBeads 40 TREN is shown in Figure 1.

 

WorkBeads 40 TREN resin can be used for the separation of biomolecules exploiting surface
charge to purify proteins, peptides and oligonucleotides.
It can also be used in flow through mode to adsorb impurities while letting the target
pass through the column (negative chromatography mode).​

 

The main characteristics of WorkBeads 40 TREN resin are shown in Table 1.
For more detailed instructions of how to use WorkBeads 40 TREN, see instruction IN 40 600 020.

 

 

Main characteristics of WorkBeads 40 TREN resin.

 

 WorkBeads 40 TREN

 Target substances

 Proteins, peptides and oligonucleotides.

 Chromatin fragments.

 Matrix

 Rigid, highly cross-linked agarose

 Average particle size1 (Dv50)

 45 μm

 Ligand

 Tris(2-ethylaminoethyl)amine (TAEA)

 Max flow rate

(20 cm bed height and 5 bar)

 600 cm/h

 Chemical stability

 Compatible with all standard aqueous buffers used for protein  purification.
 Should not be stored at low pH for prolonged time.

 pH stability

 2 - 13

 Storage

 2 to 25 °C in 20% ethanol

 

 

Product List

 Product name

 Size/Volume

 Article Number

 WorkBeads 40 TREN

 Bulk-Media - 25 mL

 40 603 001

 Bulk-Media - 150 mL

 40 603 003

 Bulk-Media - 1 L

 40 603 010