AMICOGEN

특수효소

Specialty enzyme

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특수효소 - 제약용

AMK318

Immobilized cephalexin acylase

Description
It produces cephalexin from 7-ADCA(7-amino-3-deacetoxy-cephalosporanic acid) and PGM(phenylglycine methyl ester).

 

 

Standard

 Activitya

 200 U/g

 Size of immobilization support

 300~500μm (>98%)

 Water content

 <60%

 Enzyme assay

 aOne unit (U) represents the amount of enzyme required for producing 1μmol cephalexin per min in 50mM K-phosphate buffer containing 40mM 7-ADCA(7-amino-3-deacetoxy-cephalosporanic acid) and 40mM PGM(phenylglycine methyl ester), pH 7.0 at 28℃. 

 

 

 

Application
Cephalexin production from 7-ADCA and PGM

 

 

Storage temperature
4~10°C

 

 

Characteristics
·High production of cephalexin
·Low degradation activity of cephalexin and PGM
·Fast enzyme reaction (reaction completed in 90 minutes)
·High stability (reusable more than 500 cycles)
·High production rate of cephalexin (more than 98.5%)​

 

 

Reaction condition

 pH

 7.3~8.0

 Temperature

 10°C

 Solvent

 Water

 7ADCA : PGM

 1:1.03-1.10

 Enzyme amount

(In comparison with 7ADCA)

 1:1

 

 

 

Scheme for enzyme reaction

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Optimal reaction conditions
(Temperature, pH)

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